Superpolymyxin™

PRINCIPLE This optimal screening medium is based on the eosin methylene blue (EMB) medium, which is selective for Gram negative bacteria. It contributes to a presumption of species identification by differentiating lactose fermenters (dark blue-brown colonies) from non fermenters (colorless or light lavender). Moreover, differentiation of lactose fermenters is possible with Escherichia coli colonies displaying a characteristic metallic green sheen and Enterobacter spp. and Klebsiella spp. with brown, dark-centered, and mucoid colonies. The optimal colistin concentration detects all polymyxin-resistant strains. Daptomycin is used as an inhibitor of Enterococcus and Staphylococcus strains. Amphotericin B is used as an antifungal molecule.   METHODOLOGY Samples: Bacterial colonies, rectal swabs Procedure: Direct streaking from rectal swab transport medium, preparation of a 0.5 McFarland suspension broth needed for colonies / Incubation 24-48h at 37°C / Aerobic conditions   PRODUCT SPECIFICATIONS

• 2.5 months shelf life in ready-to-use format
• No need for a selective pre-enrichment medium
• Incubation 24-48h
• No equipment required

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REAGENTS AND MATERIAL   SUPERPOLYMYXIN : 20 Agar plates sealed in plastic (2 bags each containing 10 plates) and protected from the light in cardboard packaging.   STABILITY AND STORAGE

• The SUPERPOLYMYXIN agar plates are ready-to-use. When stored at 2-8°C, in their original packaging and protected from the light, they are stable up to the expiry date.
• Agar plates from opened stacks of 10 plates can be used for 1 week when stored in a clean area at 2-8°C.
• To avoid crystallization of the medium, do not place the agar plates in direct contact with the cooling surfaces of the storage enclosure.
• To avoid the formation of condensation on the inside surface of the lid, it is recommended that the agar plate should not be subjected to large temperature variations.

PERFORMANCE The performance evaluation of SUPERPOLYMYXIN agar medium was conducted in the Microbiologie Fondamentale et Pathogénicité laboratory of the Centre National de Recherche Scientifique (CNRS UMR5234) in Bordeaux, using bacterial cultures, clinical rectal specimens and rectal specimens supplemented with Enterobacterial isolates resistant to colistin. The minimum inhibitory concentrations (MIC) were determined with the liquid micro-dilution reference method in Mueller-Hinton cation adjusted broth, as recommended by the Clinical Laboratory Standard Institute (CLSI) guide. The MIC results were interpreted according to the benchmarks of the European Committee on Antimicrobial Susceptibility Testing (EUCAST). Global Performance (based on strain analysis including all strains isolated from bacterial colonies and rectal swabs):

MATERIAL REQUIRED BUT NOT PROVIDED

• Ancillary culture media, reagents and laboratory equipment as required
• Incubator at 35°C +/- 1°C
• Sampling equipment (swab, culture medium)
• Isolation equipment (calibrated inoculation loop, etc)
• Contaminated waste container