Rapid Polymyxin™ Pseudomonas

The emergence and global spread of carbapenemase-producing Pseudomonas aeruginosa are of great concern to health services worldwide. Because these bacteria usually remain susceptible to polymyxins, interest in this old class of antibiotics has been recently renewed. However, the increasing use of colistin explains why aeruginosa strains resistant to colistin are increasingly reported worldwide. Currently available polymyxins susceptibility methods are fastidious, time-consuming (24 hours) and some methods are not reliable. They are poorly adapted to the clinical need and to the prevention of the dissemination of those multidrug resistant isolates. ELITechGroup Microbiology releases its new Rapid Polymyxin NP test proposed as a rapid, reliable and cost-effective test to detect polymyxins (polymyxin E or Colistin, polymyxin B) resistant Pseudomonas. This liquid method relies on the rapid and easy visualization of colorimetric reactions. The Rapid Polymyxin Pseudomonas test is easy-to-perform, very sensitive and specific. It can detect in only 3-4 hours polymyxin-resistant and –susceptible P. aeruginosa isolates , regardless of the molecular mechanism of polymyxins resistance. This test offers the possibility of detecting polymyxins resistance from bacterial cultures before any antimicrobial drug susceptibility testing results are obtained. Results are obtained at least 16 hours sooner with this test than with the reference broth microdilution method. It is close in performance to the reference dilution technique but much less cumbersome and is not based on diffusion of large polymyxin molecules in agar, which therefore prevents false susceptibility results.

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REAGENTS AND MATERIAL

STABILITY AND STORAGE

• All of the provided reagents are ready-to-use. The kit and the reagents when stored at 2-8°C in their original state are stable until the expiry date indicated on the box.
• The RP Medium Pseudomonas and RP NaCl are single use reagents.
• If the RP TC vial is used as a control for inoculum calibration of isolated colonies, it has to be kept until the last RP Medium Pseudomonas vial of the kit has been inoculated.
• During storage, the RP TC reagent must be protected from the light and maintained at 2-8°C.

PERFORMANCE

A multicentric performance evaluation was performed in three laboratories with bacterial isolates from different clinical samples:

• The National Research Institute (CNR) of Antibiotic Resistance, Bacteriology Laboratory, CHRU, Besançon, France,
• Emerging Antibiotic Resistance Unit (Medical and Molecular Microbiology, Department of Medicine, University of Fribourg, Switzerland) – Pr. P. Nordmann
• Research and Development Laboratory at ELITEch Microbio, Signes, France

The minimum inhibitory concentrations (MIC) of the tested isolates were determined according to the liquid micro-dilution reference method (Mueller-Hinton cation adjusted broth), according to the guidelines of the Clinical Laboratory Standard Institute (CLSI) (1,2). The percentage of clinical concordance of the RAPID POLYMYXIN PSEUDOMONAS test is 90.5% compared to the MIC method in liquid medium. The sensitivity of the test is 89.7% and the specificity is 90.8% .

A total of 148 strains were tested. The distribution panel of the tested strains is as follows:

MATERIAL REQUIRED BUT NOT PROVIDED

• Waste container for contaminated waste
• Densitometer
• Pipettes and tips
• Certified incubator at +36°C+/-2°C

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