PRODUCT
MYCOFAST® RevolutioN ATB+ (DIRECT)
Detection, enumeration, identification and complete antimicrobial susceptibility testing of urogenital mycoplasma: DIRECT METHOD
NEW GENERATION: “Expanding the scope of treatment options!”
- Extended panel of antibiotics tested (x11) !
- 100% compliance with worldwide recommendations !
The MYCOFAST® RevolutioN ATB+ is an accurate, cost-effective, easy-to-use colorimetric assay designed for the detection, enumeration, identification and antibiotic susceptibility determination of Ureaplasma urealyticum (U.u.) and Mycoplasma hominis (M.h.) from urogenital specimens. The MYCOFAST® RevolutioN ATB+ enables labs to determine susceptibility to a panel of new antibiotics, helping physicians comply with the latest therapeutic guidelines from CLSI (Clinical Laboratories Standards Institute). The addition of the MYCOFAST® RevolutioN ATB+ expands ELITechGroup’s family of mycoplasma test solutions, offering the most extensive antibiotic susceptibility determination at key clinical end-points.
- Extended panel of antibiotics tested, in full compliance with worldwide recommendations
- Complete diagnosis in an all-in-1 product
- The right pathologic threshold tested for each specimen type
- Fast results for clinicians
- Visual, colorimetric reading
Featuring ready-to-use reagents and an “all liquid” method, MYCOFAST® RevolutioN ATB+ identifies the growth of U.u. and M.h. after 24 hours of incubation. Our exclusive growth activator, the M.h. Supplement, guarantees fast and accurate results. Moreover, MYCOFAST® RevolutioN ATB+ enables the enumeration of U.u. > 103 CCU/mL which is compliant with pathological thresholds recognized for sperm and urine samples. Finally, the UMMt RevolutioN & UMMt RevolutioN AMIES transport media enable optimized sample management by preserving specimen integrity up to 72 hours.
Catalogue number(s):
| Reference | Name | Quantity |
| 00070 | MYCOFAST® RevolutioN ATB+ | 25 tests |
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Benefits
Intended use
Mycoplasmas are the smallest and simplest of the procaryotypes capable of self-reproduction (0.15 to 0.25 μm). They differ from other bacteria in their lack of a cell wall and hence a natural resistance to ß-lactams. Since mycoplasmas are relatively fragile, they will only grow in acellular cultures in the presence of various growth factors and at a constant temperature of 35 to 37 °C. Most human mycoplasmas are commensal. Of the 9 species that have been isolated from the urogenital tract, Ureaplasma urealyticum and Mycoplasma hominis are the most commonly found. U.u. and M.h. are sexually transmitted and can be pathogenic. Respiratory infections or meningitis can occur in the neonate as a result of contamination from the genital tract at birth. In adults, the infections caused by U.u. and M.h. are described in the table below: 
Conventional diagnosis is based upon culture on A7 agar plates followed by microscopical identification of U.u. (sea urchin shaped) or M.h. (fried-egg shaped) colonies. Since both U.u. and M.h. are commensal, infection can only be diagnosed through the determination of the pathological threshold, followed by precise enumeration.
MYCOFAST® RevolutioN ATB+ gives the solution and makes your urogenital diagnosis much easier.
Principle
MYCOFAST® RevolutioN ATB+ identifies U.u. and M.h. growth after 24 hours of incubation in a liquid medium. During growth, U.u. and M.h. metabolize urea and arginine respectively resulting in a color change of the medium, which contains phenol red indicator, from yellow-orange to red. Mycoplasma growth thus viewed enables:
- Enumeration of mycoplasma based on the rate of urea or arginine hydrolysis, which is proportional to the number of germs contained in the sample. U.u: > 103 and 104 UCC/ml M.h: > 104 UCC/ml
- Identification secured by checking U.u/M.h natural resistance to 2 antibiotics (Clindamycin and Erythromycin)
- U.u and M.h susceptibility testing to 6 antibiotics compliant with worldwide recommendations: Levofloxacin (LVX) 1-2-4 μg/mL , Moxifloxacin (MXF) 0.25-0.5-2-4 μg/mL , Erythromycin (E) 8-16 μg/mL, Clindamycin (CM) 0.25-0.5 μg/mL Tetracyclin (TE) 1-2-4-8 μg/mL, and Telithromycin 4μg/mL
- U.u and M.h susceptibility testing to 5 other antibiotics: Minocycline (MIN) 2µg/ml, Ofloxacin (OFX) 1µg/ml, Josamycin (JOS) 2µg/ml, Pristinamycin (PRI) 2µg/ml and Roxithromycin (ROX) 1µg/ml
Simple methodology
Easy-to-read and easy-to-interpret results
The results are read by the color obtained in the different wells. Urogenital mycoplasma growth is indicated when the medium turns red (alkaline). The medium remains yellow when no growth of urogenital mycoplasma occurs.
Reagents and material
- UMMt RevolutioN: Vial of 3 mL mycoplasma broth with antimicrobial agents and preservative solution. pH: 6.0 ± 0.1 25 Quantity: 25
- MYCOFAST® RevolutioN ATB+: Tray of 24 wells packed in an aluminium sachet with an integrated desiccant Quantity: 25
- Closing system: Protective translucent plastic tray lid Quantity: 25
Material required but not provided
- Sample collecting material (Swabs, cytobrushes, sterile containers for liquid samples)
- MYCOPLASMA Stabilizer (REF 00064)
- Pipettes and tips
- Waste container for contaminated waste
- Mineral oil
- Incubator at 37 ± 1 °C
Stability and storage
- All the reagents are ready-to-use. The vials may be stored at 2-8 °C, in their original packaging until the expiry date shown on the kit.
- The UMMt medium may be stored temporarily (3 months) at room temperature but is more stable at 2-8 °C.
- Do not freeze the reagents contained in the kit.
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